Nucleotide Sequence and Phylogenetic analysis of 2018 recent cases was performed at HVRI, Harbin China All the 14 CAV VP2 sequences showed 99% nucleotide identity for VP2 region in GeneBank. All the sequences in this study were closely related. The result showed samples and the reference sequence has common ancestor (Figure. 5) The authenticity of PCR amplification of VP2 was confirmed by the nucleotide sequencing.